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whole brain cdna  (TaKaRa)


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    Structured Review

    TaKaRa whole brain cdna
    Whole Brain Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 543 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+whole+brain+cdna/Human+Brain%2C+Whole+QUICK-Clone+cDNA/pmc12984142-228-14-17
    Average 94 stars, based on 543 article reviews
    whole brain cdna - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cloning:

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 m m non-essential amino acids, and 1 m m sodium pyruvate (Invitrogen). .. Cloning Human FBL2 cDNA (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_012157.2","term_id":"16306581","term_text":"NM_012157.2"}} NM_012157.2 ), human Skp1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_170679.1","term_id":"25777712","term_text":"NM_170679.1"}} NM_170679.1 ), and human Cullin1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_003592.2","term_id":"32307160","term_text":"NM_003592.2"}} NM_003592.2 ) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Amplification:

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 m m non-essential amino acids, and 1 m m sodium pyruvate (Invitrogen). .. Cloning Human FBL2 cDNA (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_012157.2","term_id":"16306581","term_text":"NM_012157.2"}} NM_012157.2 ), human Skp1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_170679.1","term_id":"25777712","term_text":"NM_170679.1"}} NM_170679.1 ), and human Cullin1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_003592.2","term_id":"32307160","term_text":"NM_003592.2"}} NM_003592.2 ) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 mM non-essential amino acids, and 1 mM sodium pyruvate (Invitrogen). .. Human FBL2 cDNA (GenBank accession number NM_012157.2), human Skp1 (GenBank accession number NM_170679.1), and human Cullin1 (GenBank accession number NM_003592.2) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 m m non-essential amino acids, and 1 m m sodium pyruvate (Invitrogen). .. Human FBL2 cDNA (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_012157.2","term_id":"16306581","term_text":"NM_012157.2"}} NM_012157.2 ), human Skp1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_170679.1","term_id":"25777712","term_text":"NM_170679.1"}} NM_170679.1 ), and human Cullin1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_003592.2","term_id":"32307160","term_text":"NM_003592.2"}} NM_003592.2 ) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Polymerase Chain Reaction:

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 m m non-essential amino acids, and 1 m m sodium pyruvate (Invitrogen). .. Cloning Human FBL2 cDNA (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_012157.2","term_id":"16306581","term_text":"NM_012157.2"}} NM_012157.2 ), human Skp1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_170679.1","term_id":"25777712","term_text":"NM_170679.1"}} NM_170679.1 ), and human Cullin1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_003592.2","term_id":"32307160","term_text":"NM_003592.2"}} NM_003592.2 ) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Article Title: Screening method for an agent for treatment of neurodegenerative disease
    Article Snippet: .. To amplify the human type FBL2 (hFBL2) gene, PCR was performed using synthetic primers (SEQ ID NO:19 and SEQ ID NO:4), pyrobest (Takara) as the enzyme, and a quick clone human whole brain cDNA (CLONTECH) as the template under the following conditions (1) to (3) to yield a specific PCR product. (1) 94° C. 5 minutes (2) 94° C. 30 seconds—56° C. 30 seconds—72° C. 2 minutes in 35 cycles (3) 72° C. 7 minutes The PCR product obtained was cloned into pcDNA3.1/V5-His TOPO (Invitrogen) and transformed to Escherichia coli DH5α. .. PCR was performed using the colony obtained, synthetic primers (SEQ ID NO:5 and SEQ ID NO:6) and ExTaq (Takara) as the enzyme to yield a PCR product.

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 mM non-essential amino acids, and 1 mM sodium pyruvate (Invitrogen). .. Human FBL2 cDNA (GenBank accession number NM_012157.2), human Skp1 (GenBank accession number NM_170679.1), and human Cullin1 (GenBank accession number NM_003592.2) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Article Title: srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity
    Article Snippet: Full-length human srGAP1 and srGAP2 cDNAs were purchased from Open Biosystems. .. Full-length human MRLC ( MYL12B ), RhoA , Rac1 , and Cdc42 cDNAs were obtained by PCR using human whole brain cDNA (Clontech, Mountain View, CA). .. Site-directed mutagenesis was performed using the PrimeSTAR MAX DNA polymerase according to the manufacturer's instructions (TaKaRa Bio, Otsu, Japan).

    Article Title: srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity
    Article Snippet: Full-length human srGAP1 and srGAP2 cDNAs were purchased from Open Biosystems. .. Full-length human MRLC (MYL12B), RhoA, Rac1, and Cdc42 cDNAs were obtained by PCR using human whole brain cDNA (Clontech, Mountain View, CA). .. Site-directed mutagenesis was performed using the PrimeSTAR MAX DNA polymerase according to the manufacturer’s instructions (TaKaRa Bio, Otsu, Japan).

    Article Title: FBL2 Regulates Amyloid Precursor Protein (APP) Metabolism by Promoting Ubiquitination-Dependent APP Degradation and Inhibition of APP Endocytosis
    Article Snippet: IMR-32 cells were passaged in minimum essential medium (Eagle) (Invitrogen) supplemented with 10% FBS, 0.1 m m non-essential amino acids, and 1 m m sodium pyruvate (Invitrogen). .. Human FBL2 cDNA (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_012157.2","term_id":"16306581","term_text":"NM_012157.2"}} NM_012157.2 ), human Skp1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_170679.1","term_id":"25777712","term_text":"NM_170679.1"}} NM_170679.1 ), and human Cullin1 (GenBank accession number {"type":"entrez-nucleotide","attrs":{"text":"NM_003592.2","term_id":"32307160","term_text":"NM_003592.2"}} NM_003592.2 ) were amplified by PCR from quick clone human whole-brain cDNA (Clontech). .. The cDNAs were cloned into the pcDNA3.1/V5-His TOPO vector (Invitrogen).

    Clone Assay:

    Article Title: Screening method for an agent for treatment of neurodegenerative disease
    Article Snippet: .. To amplify the human type FBL2 (hFBL2) gene, PCR was performed using synthetic primers (SEQ ID NO:19 and SEQ ID NO:4), pyrobest (Takara) as the enzyme, and a quick clone human whole brain cDNA (CLONTECH) as the template under the following conditions (1) to (3) to yield a specific PCR product. (1) 94° C. 5 minutes (2) 94° C. 30 seconds—56° C. 30 seconds—72° C. 2 minutes in 35 cycles (3) 72° C. 7 minutes The PCR product obtained was cloned into pcDNA3.1/V5-His TOPO (Invitrogen) and transformed to Escherichia coli DH5α. .. PCR was performed using the colony obtained, synthetic primers (SEQ ID NO:5 and SEQ ID NO:6) and ExTaq (Takara) as the enzyme to yield a PCR product.

    Transformation Assay:

    Article Title: Screening method for an agent for treatment of neurodegenerative disease
    Article Snippet: .. To amplify the human type FBL2 (hFBL2) gene, PCR was performed using synthetic primers (SEQ ID NO:19 and SEQ ID NO:4), pyrobest (Takara) as the enzyme, and a quick clone human whole brain cDNA (CLONTECH) as the template under the following conditions (1) to (3) to yield a specific PCR product. (1) 94° C. 5 minutes (2) 94° C. 30 seconds—56° C. 30 seconds—72° C. 2 minutes in 35 cycles (3) 72° C. 7 minutes The PCR product obtained was cloned into pcDNA3.1/V5-His TOPO (Invitrogen) and transformed to Escherichia coli DH5α. .. PCR was performed using the colony obtained, synthetic primers (SEQ ID NO:5 and SEQ ID NO:6) and ExTaq (Takara) as the enzyme to yield a PCR product.



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